1QDB

CYTOCHROME C NITRITE REDUCTASE

1QDB の概要

• エントリーDOI: 10.2210/pdb1qdb/pdb
• 分子名称: CYTOCHROME C NITRITE REDUCTASE, CALCIUM ION, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: c-type cytochrome lysine-coordinated heme nitrite reductase, oxidoreductase
• 由来する生物種: Sulfurospirillum deleyianum
• 細胞内の位置: Periplasm: Q9Z4P4
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 170391.39

構造登録者

Einsle, O.,Messerschmidt, A.,Stach, P.,Huber, R.,Kroneck, P.M.H. (登録日: 1999-05-19, 公開日: 1999-08-18, 最終更新日: 2024-11-20)

主引用文献

Einsle, O.,Messerschmidt, A.,Stach, P.,Bourenkov, G.P.,Bartunik, H.D.,Huber, R.,Kroneck, P.M.
Structure of cytochrome c nitrite reductase.
Nature, 400:476-480, 1999

PubMed Abstract: The enzyme cytochrome c nitrite reductase catalyses the six-electron reduction of nitrite to ammonia as one of the key steps in the biological nitrogen cycle, where it participates in the anaerobic energy metabolism of dissimilatory nitrate ammonification. Here we report on the crystal structure of this enzyme from the microorganism Sulfurospirillum deleyianum, which we solved by multiwavelength anomalous dispersion methods. We propose a reaction scheme for the transformation of nitrite based on structural and spectroscopic information. Cytochrome c nitrite reductase is a functional dimer, with 10 close-packed haem groups of type c and an unusual lysine-coordinated high-spin haem at the active site. By comparing the haem arrangement of this nitrite reductase with that of other multihaem cytochromes, we have been able to identify a family of proteins in which the orientation of haem groups is conserved whereas structure and function are not.

PubMed: 10440380
DOI: 10.1038/22802

実験手法

X-RAY DIFFRACTION (1.9 Å)