1QB6

BOVINE TRYPSIN 3,3'-[3,5-DIFLUORO-4-METHYL-2, 6-PYRIDINEDIYLBIS(OXY)]BIS(BENZENECARBOXIMIDAMIDE) (ZK-805623) COMPLEX

1QB6 の概要

• エントリーDOI: 10.2210/pdb1qb6/pdb
• 関連するPDBエントリー: 1FAX 1MTU 1MTW 1QAO 1QBN 1QBO
• 分子名称: PROTEIN (TRYPSIN), CALCIUM ION, POTASSIUM ION, ... (5 entities in total)
• 機能のキーワード: hydrolase, serine proteinase, protein-inhibitor complex, s1 pocket, factor xa
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted, extracellular space: P00760
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23801.85

構造登録者

Whitlow, M. (登録日: 1999-04-29, 公開日: 2000-04-29, 最終更新日: 2024-11-13)

主引用文献

Whitlow, M.,Arnaiz, D.O.,Buckman, B.O.,Davey, D.D.,Griedel, B.,Guilford, W.J.,Koovakkat, S.K.,Liang, A.,Mohan, R.,Phillips, G.B.,Seto, M.,Shaw, K.J.,Xu, W.,Zhao, Z.,Light, D.R.,Morrissey, M.M.
Crystallographic analysis of potent and selective factor Xa inhibitors complexed to bovine trypsin.
Acta Crystallogr.,Sect.D, 55:1395-1404, 1999

PubMed Abstract: Factor Xa is a serine protease which activates thrombin (factor IIa) and plays a key regulatory role in the blood-coagulation cascade. Factor Xa is, therefore, an important target for the design of anti-thrombotics. Both factor Xa and thrombin share sequence and structural homology with trypsin. As part of a factor Xa inhibitor-design program, a number of factor Xa inhibitors were crystallographically studied complexed to bovine trypsin. The structures of one diaryl benzimidazole, one diaryl carbazole and three diaryloxypyridines are described. All five compounds bind to trypsin in an extended conformation, with an amidinoaryl group in the S1 pocket and a second basic/hydrophobic moiety bound in the S4 pocket. These binding modes all bear a resemblance to the reported binding mode of DX-9065a in bovine trypsin and human factor Xa.

PubMed: 10417407
DOI: 10.1107/S0907444999007350

実験手法

X-RAY DIFFRACTION (1.8 Å)