1Q9W

S45-18 Fab pentasaccharide bisphosphate complex

1Q9W の概要

• エントリーDOI: 10.2210/pdb1q9w/pdb
• 関連するPDBエントリー: 1q9k 1q9l 1q9o 1q9q 1q9r 1q9t 1q9v
• 分子名称: S45-18 Fab (IgG1k) light chain, S45-18 Fab (IgG1k) heavy chain, 3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid-(2-4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid-(2-4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid-(2-6)-2-amino-2-deoxy-4-O-phosphono-alpha-D-glucopyranose-(1-6)-2-amino-2-deoxy-1-O-phosphono-alpha-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: antigen-binding fragment, fab, anti-cabohydrate, anti-lps, antibody, immunoglobulin, kdo, protein-carbohydrate complex, immune system
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 99463.76

構造登録者

Nguyen, H.P.,Seto, N.O.,MacKenzie, C.R.,Brade, L.,Kosma, P.,Brade, H.,Evans, S.V. (登録日: 2003-08-26, 公開日: 2004-01-27, 最終更新日: 2024-11-20)

主引用文献

Nguyen, H.P.,Seto, N.O.,MacKenzie, C.R.,Brade, L.,Kosma, P.,Brade, H.,Evans, S.V.
Germline antibody recognition of distinct carbohydrate epitopes.
Nat.Struct.Biol., 10:1019-1025, 2003

PubMed Abstract: High-resolution structures reveal how a germline antibody can recognize a range of clinically relevant carbohydrate epitopes. The germline response to a carbohydrate immunogen can be critical to survivability, with selection for antibody gene segments that both confer protection against common pathogens and retain the flexibility to adapt to new disease organisms. We show here that antibody S25-2 binds several distinct inner-core epitopes of bacterial lipopolysaccharides (LPSs) by linking an inherited monosaccharide residue binding site with a subset of complementarity-determining regions (CDRs) of limited flexibility positioned to recognize the remainder of an array of different epitopes. This strategy allows germline antibodies to adapt to different epitopes while minimizing entropic penalties associated with the immobilization of labile CDRs upon binding of antigen, and provides insight into the link between the genetic origin of individual CDRs and their respective roles in antigen recognition.

PubMed: 14625588
DOI: 10.1038/nsb1014

実験手法

X-RAY DIFFRACTION (1.75 Å)