1Q7D

Structure of the integrin alpha2beta1 binding collagen peptide

1Q7D の概要

• エントリーDOI: 10.2210/pdb1q7d/pdb
• 関連するPDBエントリー: 1dzi
• 分子名称: collagen alfa 1(I) chain peptide GPOGPOGFOGERGPOGPOGPO (2 entities in total)
• 機能のキーワード: collagen, integrin, triplehelix, contractile protein
• 細胞内の位置: Secreted, extracellular space, extracellular matrix (By similarity): P02452
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 6117.50

構造登録者

Emsley, J.,Knight, C.G.,Farndale, R.W.,Barnes, M.J. (登録日: 2003-08-18, 公開日: 2004-01-13, 最終更新日: 2025-03-26)

主引用文献

Emsley, J.,Knight, C.G.,Farndale, R.W.,Barnes, M.J.
Structure of the Integrin alpha2beta1-binding Collagen Peptide.
J.Mol.Biol., 335:1019-1028, 2004

PubMed Abstract: We have determined the 1.8A crystal structure of a triple helical integrin-binding collagen peptide (IBP) with sequence (Gly-Pro-Hyp)(2)-Gly-Phe-Hyp-Gly-Glu-Arg-(Gly-Pro-Hyp)(3). The central GFOGER hexapeptide is recognised specifically by the integrins alpha2beta1, alpha1beta1, alpha10beta1 and alpha11beta1. These integrin/collagen interactions are implicated in a number of key physiological processes including cell adhesion, cell growth and differentiation, and pathological states such as thrombosis and tumour metastasis. Comparison of the IBP structure with the previously determined structure of an identical collagen peptide in complex with the integrin alpha2-I domain (IBP(c)) allows the first detailed examination of collagen in a bound and an unbound state. The IBP structure shows a direct and a water-mediated electrostatic interaction between Glu and Arg side-chains from adjacent strands, but no intra-strand interactions. The interactions between IBP Glu and Arg side-chains are disrupted upon integrin binding. A comparison of IBP and IBP(c) main-chain conformation reveals the flexible nature of the triple helix backbone in the imino-poor GFOGER region. This flexibility could be important to the integrin-collagen interaction and provides a possible explanation for the unique orientation of the three GFOGER strands observed in the integrin-IBP(c) complex crystal structure.

PubMed: 14698296
DOI: 10.1016/j.jmb.2003.11.030

実験手法

X-RAY DIFFRACTION (1.8 Å)