1Q75

Solution structure of the dyskeratosis congenita mutant P2b hairpin from human telomerase RNA

1Q75 の概要

• エントリーDOI: 10.2210/pdb1q75/pdb
• 関連するPDBエントリー: 1NA2
• NMR情報: BMRB: 5932
• 分子名称: DKC mutant P2b telomerase RNA (1 entity in total)
• 機能のキーワード: tetraloop, pentaloop, uucg, ynmg, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4783.89

構造登録者

Theimer, C.A.,Finger, L.D.,Feigon, J. (登録日: 2003-08-15, 公開日: 2003-12-23, 最終更新日: 2024-05-22)

主引用文献

Theimer, C.A.,Finger, L.D.,Feigon, J.
YNMG tetraloop formation by a dyskeratosis congenita mutation in human telomerase RNA.
Rna, 9:1446-1455, 2003

PubMed Abstract: Autosomal dominant dyskeratosis congenita (DKC) has been linked to mutations in the RNA component of telomerase, the ribonucleoprotein responsible for telomere maintenance. Recent studies have investigated the role of the GC (107-108) --> AG mutation in the conserved P3 helix in the pseudoknot domain of human telomerase RNA. The mutation was found to significantly destabilize the pseudoknot conformation, resulting in a shift in the thermodynamic equilibrium to favor formation of a P2b hairpin intermediate. In the wild-type sequence, the hairpin intermediate was found to form a novel sequence of pyrimidine base pairs in a continuous stem capped by a structured pentaloop. The DKC mutant hairpin was observed to be slightly more stable than the wild-type hairpin, further shifting the pseudoknot-hairpin equilibrium to favor the mutant P2b hairpin. Here we examined the solution structure of the DKC mutant hairpin to identify the reason for this additional stability. We found that the mutant hairpin forms the same stem structure as wild-type and that the additional stabilization observed using optical melting can be explained by the formation of a YNMG-type tetraloop structure, with the last nucleotide of the pentaloop bulged out into the major groove. Our results provide a structural explanation for the increased stability of the mutant hairpin and further our understanding of the effect of this mutation on the structure and stability of the dominant conformation of the pseudoknot domain in this type of DKC.

PubMed: 14624001
DOI: 10.1261/rna.5152303

実験手法

SOLUTION NMR