1Q0Y

Anti-Morphine Antibody 9B1 Complexed with Morphine

1Q0Y の概要

• エントリーDOI: 10.2210/pdb1q0y/pdb
• 関連するPDBエントリー: 1Q0X
• 分子名称: Fab 9B1, Light chain, Fab 9B1, Heavy chain, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: anti-morphine antibody, fab, immune system
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46442.81

構造登録者

Pozharski, E.,Wilson, M.A.,Hewagama, A.,Shanafelt, A.B.,Petsko, G.,Ringe, D. (登録日: 2003-07-17, 公開日: 2004-04-20, 最終更新日: 2024-10-16)

主引用文献

Pozharski, E.,Wilson, M.A.,Hewagama, A.,Shanafelt, A.B.,Petsko, G.,Ringe, D.
Anchoring a cationic ligand: the structure of the Fab fragment of the anti-morphine antibody 9B1 and its complex with morphine
J.Mol.Biol., 337:691-697, 2004

PubMed Abstract: The crystal structures of an anti-morphine antibody 9B1 (to 1.6A resolution) and its complex with morphine (to 2.0 A resolution) are reported. The morphine-binding site is described as a shallow depression on the protein surface, an unusual topology for a high-affinity ( Ka approximately 10(9) M(-1)) antibody against a small antigen. The polar part of the ligand is exposed to solvent, and the cationic nitrogen atom of the morphine molecule is anchored at the bottom of the binding site by a salt-bridge to a glutamate side-chain. Additional affinity is provided by a double cation-pi interaction with two tryptophan residues. Comparison of the morphine complex with the structure of the free Fab shows that a domain closure occurs upon binding of the ligand.

PubMed: 15019787
DOI: 10.1016/j.jmb.2003.12.084

実験手法

X-RAY DIFFRACTION (2 Å)