1PUS

Solution Structure of the MutT Pyrophosphohydrolase Complexed with Mg(2+) and 8-oxo-dGMP, a Tightly-bound Product

1PUS の概要

• エントリーDOI: 10.2210/pdb1pus/pdb
• 関連するPDBエントリー: 1MUT 1PPX 1PUN 1PUQ 1TUM
• 分子名称: Mutator mutT protein, MAGNESIUM ION, 8-OXO-2'-DEOXY-GUANOSINE-5'-MONOPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: mutator protein, nucleoside triphosphate pyrophosphohydrolase, mutt pyrophosphohydrolase-metal-product complex, hydrolase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15332.56

構造登録者

Massiah, M.A.,Saraswat, V.,Azurmendi, H.F.,Mildvan, A.S. (登録日: 2003-06-25, 公開日: 2003-08-26, 最終更新日: 2024-05-01)

主引用文献

Massiah, M.A.,Saraswat, V.,Azurmendi, H.F.,Mildvan, A.S.
Solution structure and NH exchange studies of the MutT pyrophosphohydrolase complexed with Mg(2+) and 8-oxo-dGMP, a tightly bound product.
Biochemistry, 42:10140-10154, 2003

PubMed Abstract: To learn the structural basis for the unusually tight binding of 8-oxo-nucleotides to the MutT pyrophosphohydrolase of Escherichia coli (129 residues), the solution structure of the MutT-Mg(2+)-8-oxo-dGMP product complex (K(D) = 52 nM) was determined by standard 3-D heteronuclear NMR methods. Using 1746 NOEs (13.5 NOEs/residue) and 186 phi and psi values derived from backbone (15)N, Calpha, Halpha, and Cbeta chemical shifts, 20 converged structures were computed with NOE violations PubMed: 12939141
DOI: 10.1021/bi030105p

実験手法

SOLUTION NMR