1PTR

PROTEIN KINASE C DELTA CYS2 DOMAIN COMPLEXED WITH PHORBOL-13-ACETATE

1PTR の概要

• エントリーDOI: 10.2210/pdb1ptr/pdb
• 分子名称: PROTEIN KINASE C DELTA TYPE, ZINC ION, 13-ACETYLPHORBOL (3 entities in total)
• 機能のキーワード: phosphotransferase
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cytoplasm (By similarity): P28867
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6349.14

構造登録者

Zhang, G.,Hurley, J.H. (登録日: 1995-05-11, 公開日: 1995-07-31, 最終更新日: 2024-02-14)

主引用文献

Zhang, G.,Kazanietz, M.G.,Blumberg, P.M.,Hurley, J.H.
Crystal structure of the cys2 activator-binding domain of protein kinase C delta in complex with phorbol ester.
Cell(Cambridge,Mass.), 81:917-924, 1995

PubMed Abstract: Protein kinase Cs (PKCs) are a ubiquitous family of regulatory enzymes that associate with membranes and are activated by diacylglycerol or tumor-promoting agonists such as phorbol esters. The structure of the second activator-binding domain of PKC delta has been determined in complex with phorbol 13-acetate, which binds in a groove between two pulled-apart beta strands at the tip of the domain. The C3, C4, and C20 phorbol oxygens form hydrogen bonds with main-chain groups whose orientation is controlled by a set of highly conserved residues. Phorbol binding caps the groove and forms a contiguous hydrophobic surface covering one-third of the domain, explaining how the activator promotes insertion of PKC into membranes.

PubMed: 7781068
DOI: 10.1016/0092-8674(95)90011-X

実験手法

X-RAY DIFFRACTION (2.2 Å)