1PTQ

PROTEIN KINASE C DELTA CYS2 DOMAIN

Summary for 1PTQ

• Entry DOI: 10.2210/pdb1ptq/pdb
• Descriptor: PROTEIN KINASE C DELTA TYPE, ZINC ION (2 entities in total)
• Functional Keywords: phosphotransferase
• Biological source: Mus musculus (house mouse)
• Cellular location: Cytoplasm (By similarity): P28867
• Total number of polymer chains: 1
• Total formula weight: 5942.67

Authors

Zhang, G.,Hurley, J.H. (deposition date: 1995-05-11, release date: 1995-07-31, Last modification date: 2024-02-14)

Primary citation

Zhang, G.,Kazanietz, M.G.,Blumberg, P.M.,Hurley, J.H.
Crystal structure of the cys2 activator-binding domain of protein kinase C delta in complex with phorbol ester.
Cell(Cambridge,Mass.), 81:917-924, 1995

PubMed Abstract: Protein kinase Cs (PKCs) are a ubiquitous family of regulatory enzymes that associate with membranes and are activated by diacylglycerol or tumor-promoting agonists such as phorbol esters. The structure of the second activator-binding domain of PKC delta has been determined in complex with phorbol 13-acetate, which binds in a groove between two pulled-apart beta strands at the tip of the domain. The C3, C4, and C20 phorbol oxygens form hydrogen bonds with main-chain groups whose orientation is controlled by a set of highly conserved residues. Phorbol binding caps the groove and forms a contiguous hydrophobic surface covering one-third of the domain, explaining how the activator promotes insertion of PKC into membranes.

PubMed: 7781068
DOI: 10.1016/0092-8674(95)90011-X

Experimental method

X-RAY DIFFRACTION (1.95 Å)