1PS9

The Crystal Structure and Reaction Mechanism of E. coli 2,4-Dienoyl CoA Reductase

1PS9 の概要

• エントリーDOI: 10.2210/pdb1ps9/pdb
• 分子名称: 2,4-dienoyl-CoA reductase, CHLORIDE ION, IRON/SULFUR CLUSTER, ... (8 entities in total)
• 機能のキーワード: iron-sulfur, tim barrel, flavodoxin, flavin, electron transfer, hydride transfer, oxidoreductase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 76065.89

構造登録者

Hubbard, P.A.,Liang, X.,Schulz, H.,Kim, J.J. (登録日: 2003-06-20, 公開日: 2003-09-30, 最終更新日: 2024-02-14)

主引用文献

Hubbard, P.A.,Liang, X.,Schulz, H.,Kim, J.J.
The crystal structure and reaction mechanism of Escherichia coli 2,4-dienoyl-CoA reductase
J.Biol.Chem., 278:37553-37560, 2003

PubMed Abstract: Escherichia coli 2,4-dienoyl-CoA reductase is an iron-sulfur flavoenzyme required for the metabolism of unsaturated fatty acids with double bonds at even carbon positions. The enzyme contains FMN, FAD, and a 4Fe-4S cluster and exhibits sequence homology to another iron-sulfur flavoprotein, trimethylamine dehydrogenase. It also requires NADPH as an electron source, resulting in reduction of the C4-C5 double bond of the acyl chain of the CoA thioester substrate. The structure presented here of a ternary complex of E. coli 2,4-dienoyl-CoA reductase with NADP+ and a fatty acyl-CoA substrate reveals a possible mechanism for substrate reduction and provides details of a plausible electron transfer mechanism involving both flavins and the iron-sulfur cluster. The reaction is initiated by hydride transfer from NADPH to FAD, which in turn transfers electrons, one at a time, to FMN via the 4Fe-4S cluster. In the final stages of the reaction, the fully reduced FMN provides a hydride ion to the C5 atom of substrate, and Tyr-166 and His-252 are proposed to form a catalytic dyad that protonates the C4 atom of the substrate and complete the reaction. Inspection of the substrate binding pocket explains the relative promiscuity of the enzyme, catalyzing reduction of both 2-trans,4-cis- and 2-trans,4-trans-dienoyl-CoA thioesters.

PubMed: 12840019
DOI: 10.1074/jbc.M304642200

実験手法

X-RAY DIFFRACTION (2.2 Å)