1PP4

The crystal structure of rhamnogalacturonan acetylesterase in space group P3121

1PP4 の概要

• エントリーDOI: 10.2210/pdb1pp4/pdb
• 関連するPDBエントリー: 1DEO 1DEX 1K7C
• 分子名称: Rhamnogalacturonan acetylesterase, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: gds(l) hydrolase, hydrolase
• 由来する生物種: Aspergillus aculeatus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 50130.59

構造登録者

Molgaard, A.,Larsen, S. (登録日: 2003-06-16, 公開日: 2004-03-02, 最終更新日: 2024-10-16)

主引用文献

Molgaard, A.,Larsen, S.
Crystal packing in two pH-dependent crystal forms of rhamnogalacturonan acetylesterase.
Acta Crystallogr.,Sect.D, 60:472-478, 2004

PubMed Abstract: The glycoprotein rhamnogalacturonan acetylesterase from Aspergillus aculeatus has been crystallized in two crystal forms, an orthorhombic and a trigonal crystal form. In the orthorhombic crystal form, the covalently bound carbohydrate at one of the two N-glycosylation sites is involved in crystal contacts. The orthorhombic crystal form was obtained at pH 5.0 and the trigonal crystal form at pH 4.5. In one case, the two crystal forms were found in the same drop at pH 4.7. The differences in crystal packing in the two crystal forms can be explained by the pH-dependent variation in the protonation state of the glutamic acid residues on the protein surface.

PubMed: 14993671
DOI: 10.1107/S0907444903029767

実験手法

X-RAY DIFFRACTION (2.5 Å)