1PP1

Crystal structure of the Borna Disease Virus Nucleoprotein

1PP1 の概要

• エントリーDOI: 10.2210/pdb1pp1/pdb
• 関連するPDBエントリー: 1n93
• 分子名称: p40 nucleoprotein (2 entities in total)
• 機能のキーワード: rna virus nucleoprotein tetramer, viral protein
• 由来する生物種: Borna disease virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42072.26

構造登録者

Rudolph, M.G.,Kraus, I.,Dickmanns, A.,Garten, W.,Ficner, R. (登録日: 2003-06-16, 公開日: 2003-10-07, 最終更新日: 2024-11-13)

主引用文献

Rudolph, M.G.,Kraus, I.,Dickmanns, A.,Eickmann, M.,Garten, W.,Ficner, R.
Crystal structure of the Borna Disease Virus nucleoprotein
Structure, 11:1219-1226, 2003

PubMed Abstract: Borna disease virus (BDV) causes an infection of the central nervous system in a wide range of vertebrates, which can fatally progress to an immune-mediated disease, called Borna disease. BDV is a member of the Mononegavirales, which also includes the highly infectious measles and Ebola viruses. The viral nucleoproteins are central to transcription, replication, and packaging of the RNA genome. We present the X-ray structure of the BDV nucleoprotein determined at 1.76 A resolution. The structure reveals a novel fold, organized into two distinct domains, and an assembly into a planar homotetramer. Surface potential calculations strongly support an RNA binding model with the RNA wrapping around the outside of the tetramer, although a positively charged central channel in the tetramer could fit single-stranded RNA in an alternative binding mode. This first structure of an RNA virus nucleoprotein provides a paradigmatic model for RNA packaging and replication of single-stranded RNA viruses.

PubMed: 14527390
DOI: 10.1016/j.str.2003.08.011

実験手法

X-RAY DIFFRACTION (1.9 Å)