1POI

CRYSTAL STRUCTURE OF GLUTACONATE COENZYME A-TRANSFERASE FROM ACIDAMINOCOCCUS FERMENTANS TO 2.55 ANGSTOMS RESOLUTION

1POI の概要

• エントリーDOI: 10.2210/pdb1poi/pdb
• 分子名称: GLUTACONATE COENZYME A-TRANSFERASE, COPPER (II) ION, ... (4 entities in total)
• 機能のキーワード: transferase, coa, glutamate, protein fermentation
• 由来する生物種: Acidaminococcus fermentans; 詳細
• 細胞内の位置: Cytoplasm: Q59111 Q59112
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 127977.19

構造登録者

Jacob, U.,Mack, M.,Clausen, T.,Huber, R.,Buckel, W.,Messerschmidt, A. (登録日: 1997-01-24, 公開日: 1998-03-18, 最終更新日: 2024-02-14)

主引用文献

Jacob, U.,Mack, M.,Clausen, T.,Huber, R.,Buckel, W.,Messerschmidt, A.
Glutaconate CoA-transferase from Acidaminococcus fermentans: the crystal structure reveals homology with other CoA-transferases.
Structure, 5:415-426, 1997

PubMed Abstract: Coenzyme A-transferases are a family of enzymes with a diverse substrate specificity and subunit composition. Members of this group of enzymes are found in anaerobic fermenting bacteria, aerobic bacteria and in the mitochondria of humans and other mammals, but so far none have been crystallized. A defect in the human gene encoding succinyl-CoA: 3-oxoacid CoA-transferase causes a metabolic disease which leads to severe ketoacidosis, thus reflecting the importance of this family of enzymes. All CoA-transferases share a common mechanism in which the CoA moiety is transferred from a donor (e.g. acetyl CoA) to an acceptor, (R)-2-hydroxyglutarate, whereby acetate is formed. The transfer has been described by a ping-pong mechanism in which CoA is bound to the active-site residue of the enzyme as a covalent thiol ester intermediate. We describe here the crystal structure of glutaconate CoA-transferase (GCT) from the strictly anaerobic bacterium Acidaminococcus fermentans. This enzyme activates (R)-2-hydroxyglutarate to (R)-2-hydroxyglutaryl-CoA in the pathway of glutamate fermentation. We initiated this project to gain further insight into the function of this enzyme and the structural basis for the characteristics of CoA-transferases.

PubMed: 9083111
DOI: 10.1016/S0969-2126(97)00198-6

実験手法

X-RAY DIFFRACTION (2.5 Å)