1POC

CRYSTAL STRUCTURE OF BEE-VENOM PHOSPHOLIPASE A2 IN A COMPLEX WITH A TRANSITION-STATE ANALOGUE

1POC の概要

• エントリーDOI: 10.2210/pdb1poc/pdb
• 分子名称: PHOSPHOLIPASE A2, CALCIUM ION, 1-O-OCTYL-2-HEPTYLPHOSPHONYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE, ... (4 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Apis mellifera (honey bee)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15803.92

構造登録者

Scott, D.L.,Otwinowski, Z.,Sigler, P.B. (登録日: 1992-09-07, 公開日: 1993-10-31, 最終更新日: 2024-10-30)

主引用文献

Scott, D.L.,Otwinowski, Z.,Gelb, M.H.,Sigler, P.B.
Crystal structure of bee-venom phospholipase A2 in a complex with a transition-state analogue.
Science, 250:1563-1566, 1990

PubMed Abstract: The 2.0 angstroms crystal structure of a complex containing bee-venom phospholipase A2 (PLA2) and a phosphonate transition-state analogue was solved by multiple isomorphous replacement. The electron-density map is sufficiently detailed to visualize the proximal sugars of the enzyme's N-linked carbohydrate and a single molecule of the transition-state analogue bound ot its active center. Although bee-venom PLA2 does not belong to the large homologous Class I/II family that encompasses most other well-studied PLA2s, there is segmental sequence similarity and conservation of many functional substructures. Comparison of the bee-venom enzyme with other phospholipase structures provides compelling evidence for a common catalytic mechanism.

PubMed: 2274788

実験手法

X-RAY DIFFRACTION (2 Å)