1PNN

PEPTIDE NUCLEIC ACID (PNA) COMPLEXED WITH DNA

1PNN の概要

• エントリーDOI: 10.2210/pdb1pnn/pdb
• 分子名称: PNA (NH2-P(*C*T*C*T*T*C*T*T*C-HIS-GLY-SER-SER-GLY-HIS-C*T*T*C*T*T*C*T*C)-COOH), DNA (5'-D(GP*AP*AP*GP*AP*AP*GP*AP*G)-3') (3 entities in total)
• 機能のキーワード: hairpin pna:dna triplex, triplex watson-crick hoogsteen, peptide nucleic acid-dna complex, peptide nucleic acid/dna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 16319.94

構造登録者

Betts, L.,Veal, J.M. (登録日: 1995-10-13, 公開日: 1996-03-08, 最終更新日: 2023-11-15)

主引用文献

Betts, L.,Josey, J.A.,Veal, J.M.,Jordan, S.R.
A Nucleic Acid Triple Helix Formed by a Peptide Nucleic Acid-DNA Complex
Science, 270:1838-1841, 1995

PubMed Abstract: The crystal structure of a nucleic acid triplex reveals a helix, designated P-form, that differs from previously reported nucleic acid structures. The triplex consists of one polypurine DNA strand complexed to a polypyrimidine hairpin peptide nucleic acid (PNA) and was successfully designed to promote Watson-Crick and Hoogsteen base pairing. The P-form helix is underwound, with a base tilt similar to B-form DNA. The bases are displaced from the helix axis even more than in A-form DNA. Hydrogen bonds between the DNA backbone and the Hoogsteen PNA backbone explain the observation that polypyrimidine PNA sequences form highly stable 2:1 PNA-DNA complexes. This structure expands the number of known stable helical forms that nucleic acids can adopt.

PubMed: 8525381

実験手法

X-RAY DIFFRACTION (2.5 Å)