1PN8

Coordinates of S12, L11 proteins and E-site tRNA from 70S crystal structure separately fitted into the Cryo-EM map of E.coli 70S.EF-G.GDPNP complex. The atomic coordinates originally from the E-site tRNA were fitted in the position of the hybrid P/E-site tRNA.

Summary for 1PN8

• Entry DOI: 10.2210/pdb1pn8/pdb
• Related: 1GIX 1GIY 1PN6 1PN7
• EMDB information: 1055 1362 1363 1364 1365 1366
• Descriptor: E-tRNA, 30S ribosomal protein S12, 50S ribosomal protein L11 (3 entities in total)
• Functional Keywords: ribosomal protein, trna binding protein, trna, rna binding protein-rna complex, rna binding protein/rna
• Biological source: Thermus thermophilus; More
• Total number of polymer chains: 3
• Total formula weight: 49943.19

Authors

Valle, M.,Zavialov, A.,Sengupta, J.,Rawat, U.,Ehrenberg, M.,Frank, J. (deposition date: 2003-06-12, release date: 2003-07-15, Last modification date: 2024-02-14)

Primary citation

Valle, M.,Zavialov, A.,Sengupta, J.,Rawat, U.,Ehrenberg, M.,Frank, J.
Locking and Unlocking of Ribosomal Motions
Cell(Cambridge,Mass.), 114:123-134, 2003

PubMed Abstract: During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation is accompanied by a 20 A movement of the L1 stalk of the 50S subunit, implying that this region is involved in the translocation of deacylated tRNAs from the P to the E site. These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions.

PubMed: 12859903
DOI: 10.1016/S0092-8674(03)00476-8

Experimental method

ELECTRON MICROSCOPY (10.8 Å)