1PLR

CRYSTAL STRUCTURE OF THE EUKARYOTIC DNA POLYMERASE PROCESSIVITY FACTOR PCNA

1PLR の概要

• エントリーDOI: 10.2210/pdb1plr/pdb
• 分子名称: PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) (2 entities in total)
• 機能のキーワード: dna-binding, nuclear protein, dna replication
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Nucleus: P15873
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28944.05

構造登録者

Krishna, T.S.R.,Kong, X.-P.,Gary, S.,Burgers, P.M.,Kuriyan, J. (登録日: 1995-01-02, 公開日: 1995-03-31, 最終更新日: 2024-10-23)

主引用文献

Krishna, T.S.,Kong, X.P.,Gary, S.,Burgers, P.M.,Kuriyan, J.
Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA.
Cell(Cambridge,Mass.), 79:1233-1243, 1994

PubMed Abstract: The crystal structure of the processivity factor required by eukaryotic DNA polymerase delta, proliferating cell nuclear antigen (PCNA) from S. cerevisiae, has been determined at 2.3 A resolution. Three PCNA molecules, each containing two topologically identical domains, are tightly associated to form a closed ring. The dimensions and electrostatic properties of the ring suggest that PCNA encircles duplex DNA, providing a DNA-bound platform for the attachment of the polymerase. The trimeric PCNA ring is strikingly similar to the dimeric ring formed by the beta subunit (processivity factor) of E. coli DNA polymerase III holoenzyme, with which it shares no significant sequence identity. This structural correspondence further substantiates the mechanistic connection between eukaryotic and prokaryotic DNA replication that has been suggested on biochemical grounds.

PubMed: 8001157
DOI: 10.1016/0092-8674(94)90014-0

実験手法

X-RAY DIFFRACTION (3 Å)