1PKU

Crystal Structure of Nucleoside Diphosphate Kinase from Rice

1PKU の概要

• エントリーDOI: 10.2210/pdb1pku/pdb
• 分子名称: Nucleoside Diphosphate Kinase I (2 entities in total)
• 機能のキーワード: nucleoside diphosphate kinase, rice, transferase
• 由来する生物種: Oryza sativa (rice)
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 204473.88

構造登録者

Huang, J.-Y.,Chang, C.-Y.,Chang, T.,Chen, C.-J. (登録日: 2003-06-06, 公開日: 2005-02-22, 最終更新日: 2024-03-13)

主引用文献

Huang, J.Y.,Chang, T.,Chang, C.Y.,Chen, C.J.
Crystal structure of nucleoside diphosphate kinase required for coleoptile elongation in rice (Oryza sativa L.).
J.Struct.Biol., 150:309-318, 2005

PubMed Abstract: Nucleoside diphosphate kinase (NDK) is a ubiquitous enzyme found in all organisms and cell types, and catalyzes the transfer of the phosphoryl group from a nucleoside triphosphate to a nucleoside diphosphate. The enzyme is involved in and required for coleoptile elongation in rice as the level of the rice NDK (rNDK) changes during seed germination and the early stages of seedling growth. The expression of rice NDK gene is up-regulated in the growing coleoptiles when the anaerobic stress persists. The rNDK structure determined at 2.5 A resolution consists of a four-stranded anti-parallel beta-sheet, of which the surfaces are partially covered with six alpha-helices; its overall and active site structures are similar to those of homologous enzymes except the major conformation variations of residue 132-138 regions, involving significant structural contacts. The model contains 148 residues of 149 residues in total and averaged 19 water molecules per monomer for 12 molecules in an asymmetric unit. A mold of 12 superimposed molecules shows that the alphaA-alpha2 area has greater variations and higher temperature factors, indicating the flexibility for a substrate entrance. Hexameric molecular packing in both crystal and solution implies that rNDK functions as hexamers. This rNDK structure, which is the first NDK structure from a higher plant system, provides the structural information essential to understand the functional significance of this enzyme during growth and development in both rice and other plants.

PubMed: 15890279
DOI: 10.1016/j.jsb.2005.02.010

実験手法

X-RAY DIFFRACTION (2.5 Å)