1PKP

THE STRUCTURE OF RIBOSOMAL PROTEIN S5 REVEALS SITES OF INTERACTION WITH 16S RRNA

1PKP の概要

• エントリーDOI: 10.2210/pdb1pkp/pdb
• 分子名称: RIBOSOMAL PROTEIN S5 (1 entity in total)
• 機能のキーワード: ribosomal protein
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15914.58

構造登録者

Ramakrishnan, V.,White, S.W. (登録日: 1993-08-30, 公開日: 1994-01-31, 最終更新日: 2024-02-14)

主引用文献

Ramakrishnan, V.,White, S.W.
The structure of ribosomal protein S5 reveals sites of interaction with 16S rRNA.
Nature, 358:768-771, 1992

PubMed Abstract: Understanding the process whereby the ribosome translates the genetic code into protein molecules will ultimately require high-resolution structural information, and we report here the first crystal structure of a protein from the small ribosomal subunit. This protein, S5, has a molecular mass of 17,500 and is highly conserved in all lifeforms. The molecule contains two distinct alpha/beta domains that have structural similarities to several other proteins that are components of ribonucleoprotein complexes. Mutations in S5 result in several phenotypes which suggest that S5 may have a role in translational fidelity and translocation. These include ribosome ambiguity or ram, reversion from streptomycin dependence and resistance to spectinomycin. Also, a cold-sensitive, spectinomycin-resistant mutant of S5 has been identified which is defective in initiation. Here we show that these mutations map to two distinct regions of the molecule which seem to be sites of interaction with ribosomal RNA. A structure/function analysis of the molecule reveals discrepancies with current models of the 30S subunit.

PubMed: 1508272
DOI: 10.1038/358768a0

実験手法

X-RAY DIFFRACTION (2.8 Å)