1PJW

Solution Structure of the Domain III of the Japan Encephalitis Virus Envelope Protein

Summary for 1PJW

• Entry DOI: 10.2210/pdb1pjw/pdb
• Descriptor: envelope protein (1 entity in total)
• Functional Keywords: flavivirus, jev, e protein, epitope mapping, viral protein
• Biological source: Japanese encephalitis virus
• Cellular location: Envelope protein E: Virion membrane; Multi- pass membrane protein: Q9J0X3
• Total number of polymer chains: 1
• Total formula weight: 11769.37

Authors

Wu, K.P.,Wu, C.W.,Tsao, Y.P.,Lou, Y.C.,Lin, C.W. (deposition date: 2003-06-04, release date: 2003-11-25, Last modification date: 2024-11-06)

Primary citation

Wu, K.P.,Wu, C.W.,Tsao, Y.P.,Kuo, T.W.,Lou, Y.C.,Lin, C.W.,Wu, S.C.,Cheng, J.W.
Structural Basis of a Flavivirus Recognized by Its Neutralizing Antibody: SOLUTION STRUCTURE OF THE DOMAIN III OF THE JAPANESE ENCEPHALITIS VIRUS ENVELOPE PROTEIN.
J.Biol.Chem., 278:46007-46013, 2003

PubMed Abstract: The flavivirus envelope protein is the dominant antigen in eliciting neutralizing antibodies and plays an important role in inducing immunologic responses in the infected host. We have determined the solution structure of the major antigenic domain (domain III) of the Japanese encephalitis virus (JEV) envelope protein. The JEV domain III forms a beta-barrel type structure composed of six antiparallel beta-strands resembling the immunoglobulin constant domain. We have also identified epitopes of the JEV domain III to its neutralizing antibody by chemical shift perturbation measurements. Site-directed mutagenesis experiments are performed to confirm the NMR results. Our study provides a structural basis for understanding the mechanism of immunologic protection and for rational design of vaccines effective against flaviviruses.

PubMed: 12952958
DOI: 10.1074/jbc.M307776200

Experimental method

SOLUTION NMR