1PJW

Solution Structure of the Domain III of the Japan Encephalitis Virus Envelope Protein

1PJW の概要

• エントリーDOI: 10.2210/pdb1pjw/pdb
• 分子名称: envelope protein (1 entity in total)
• 機能のキーワード: flavivirus, jev, e protein, epitope mapping, viral protein
• 由来する生物種: Japanese encephalitis virus
• 細胞内の位置: Envelope protein E: Virion membrane; Multi- pass membrane protein: Q9J0X3
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11769.37

構造登録者

Wu, K.P.,Wu, C.W.,Tsao, Y.P.,Lou, Y.C.,Lin, C.W. (登録日: 2003-06-04, 公開日: 2003-11-25, 最終更新日: 2024-11-06)

主引用文献

Wu, K.P.,Wu, C.W.,Tsao, Y.P.,Kuo, T.W.,Lou, Y.C.,Lin, C.W.,Wu, S.C.,Cheng, J.W.
Structural Basis of a Flavivirus Recognized by Its Neutralizing Antibody: SOLUTION STRUCTURE OF THE DOMAIN III OF THE JAPANESE ENCEPHALITIS VIRUS ENVELOPE PROTEIN.
J.Biol.Chem., 278:46007-46013, 2003

PubMed Abstract: The flavivirus envelope protein is the dominant antigen in eliciting neutralizing antibodies and plays an important role in inducing immunologic responses in the infected host. We have determined the solution structure of the major antigenic domain (domain III) of the Japanese encephalitis virus (JEV) envelope protein. The JEV domain III forms a beta-barrel type structure composed of six antiparallel beta-strands resembling the immunoglobulin constant domain. We have also identified epitopes of the JEV domain III to its neutralizing antibody by chemical shift perturbation measurements. Site-directed mutagenesis experiments are performed to confirm the NMR results. Our study provides a structural basis for understanding the mechanism of immunologic protection and for rational design of vaccines effective against flaviviruses.

PubMed: 12952958
DOI: 10.1074/jbc.M307776200

実験手法

SOLUTION NMR