1PJN

Mouse Importin alpha-bipartite NLS N1N2 from Xenopus laevis phosphoprotein Complex

1PJN の概要

• エントリーDOI: 10.2210/pdb1pjn/pdb
• 関連するPDBエントリー: 1EJL 1EJY 1IQ1
• 分子名称: Histone-binding protein N1/N2, Importin alpha-2 subunit (3 entities in total)
• 機能のキーワード: importin alpha/karyopherin alpha, nuclear localization sequence (nls) recognition, bipartite nls, xenopus laevis n1n2 phosphoprotein, protein transport
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Nucleus: P52293; Cytoplasm (By similarity): P06180
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52368.57

構造登録者

Fontes, M.R.M.,Teh, T.,Jans, D.,Brinkworth, R.I.,Kobe, B. (登録日: 2003-06-03, 公開日: 2003-08-19, 最終更新日: 2023-08-16)

主引用文献

Fontes, M.R.M.,Teh, T.,Jans, D.,Brinkworth, R.I.,Kobe, B.
Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha
J.Biol.Chem., 278:27981-27987, 2003

PubMed Abstract: Importin-alpha is the nuclear import receptor that recognizes cargo proteins carrying conventional basic monopartite and bipartite nuclear localization sequences (NLSs) and facilitates their transport into the nucleus. Bipartite NLSs contain two clusters of basic residues, connected by linkers of variable lengths. To determine the structural basis of the recognition of diverse bipartite NLSs by mammalian importin-alpha, we co-crystallized a non-autoinhibited mouse receptor protein with peptides corresponding to the NLSs from human retinoblastoma protein and Xenopus laevis phosphoprotein N1N2, containing diverse sequences and lengths of the linker. We show that the basic clusters interact analogously in both NLSs, but the linker sequences adopt different conformations, whereas both make specific contacts with the receptor. The available data allow us to draw general conclusions about the specificity of NLS binding by importin-alpha and facilitate an improved definition of the consensus sequence of a conventional basic/bipartite NLS (KRX10-12KRRK) that can be used to identify novel nuclear proteins.

PubMed: 12695505
DOI: 10.1074/jbc.M303275200

実験手法

X-RAY DIFFRACTION (2.5 Å)