1PHO

CRYSTAL STRUCTURES EXPLAIN FUNCTIONAL PROPERTIES OF TWO E. COLI PORINS

1PHO の概要

• エントリーDOI: 10.2210/pdb1pho/pdb
• 分子名称: PHOSPHOPORIN (1 entity in total)
• 機能のキーワード: outer membrane protein
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell outer membrane; Multi-pass membrane protein: P02932
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36871.25

構造登録者

Schirmer, T.,Cowan, S.W.,Jansonius, J.N. (登録日: 1993-01-15, 公開日: 1993-10-31, 最終更新日: 2024-02-14)

主引用文献

Cowan, S.W.,Schirmer, T.,Rummel, G.,Steiert, M.,Ghosh, R.,Pauptit, R.A.,Jansonius, J.N.,Rosenbusch, J.P.
Crystal structures explain functional properties of two E. coli porins.
Nature, 358:727-733, 1992

PubMed Abstract: Porins form aqueous channels that aid the diffusion of small hydrophilic molecules across the outer membrane of Gram-negative bacteria. The crystal structures of matrix porin and phosphoporin both reveal trimers of identical subunits, each subunit consisting of a 16-stranded anti-parallel beta-barrel containing a pore. A long loop inside the barrel contributes to a constriction of the channel where the charge distribution affects ion selectivity. The structures explain at the molecular level functional characteristics and their alterations by known mutations.

PubMed: 1380671
DOI: 10.1038/358727a0

実験手法

X-RAY DIFFRACTION (3 Å)