1PGP

CRYSTALLOGRAPHIC STUDY OF COENZYME, COENZYME ANALOGUE AND SUBSTRATE BINDING IN 6-PHOSPHOGLUCONATE DEHYDROGENASE: IMPLICATIONS FOR NADP SPECIFICITY AND THE ENZYME MECHANISM

1PGP の概要

• エントリーDOI: 10.2210/pdb1pgp/pdb
• 関連するPDBエントリー: 1PGN 1PGO 1PGQ 2PGD
• 分子名称: 6-PHOSPHOGLUCONATE DEHYDROGENASE, 6-PHOSPHOGLUCONIC ACID, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: oxidoreductase (choh(d)-nadp+(a))
• 由来する生物種: Ovis aries (sheep)
• 細胞内の位置: Cytoplasm : P00349
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53277.85

構造登録者

Adams, M.J.,Phillips, C.,Gover, S.,Naylor, C.E. (登録日: 1994-07-18, 公開日: 1995-02-27, 最終更新日: 2024-02-14)

主引用文献

Adams, M.J.,Ellis, G.H.,Gover, S.,Naylor, C.E.,Phillips, C.
Crystallographic study of coenzyme, coenzyme analogue and substrate binding in 6-phosphogluconate dehydrogenase: implications for NADP specificity and the enzyme mechanism.
Structure, 2:651-668, 1994

PubMed Abstract: The nicotinamide adenine dinucleotide phosphate (NADP)-dependent oxidative decarboxylase, 6-phosphogluconate dehydrogenase, is a major source of reduced coenzyme for synthesis. Enzymes later in the pentose phosphate pathway convert the reaction product, ribulose 5-phosphate, to ribose 5-phosphate. Crystallographic study of complexes with coenzyme and substrate explain the NADP dependence which determines the enzyme's metabolic role and support the proposed general base-general acid mechanism.

PubMed: 7922042
DOI: 10.1016/S0969-2126(00)00066-6

実験手法

X-RAY DIFFRACTION (2.5 Å)