1PFI

PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES

1PFI の概要

• エントリーDOI: 10.2210/pdb1pfi/pdb
• 分子名称: MAJOR COAT PROTEIN OF PF1 VIRUS, DNA (5'-D(P*C)-3') (2 entities in total)
• 機能のキーワード: complex(viral coat protein-dna), helical virus, virus
• 由来する生物種: Pseudomonas phage Pf1; 詳細
• 細胞内の位置: Virion (Potential): P03621
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 9839.18

構造登録者

Liu, D.J.,Day, L.A. (登録日: 1994-04-06, 公開日: 1995-01-26, 最終更新日: 2024-02-14)

主引用文献

Liu, D.J.,Day, L.A.
Pf1 virus structure: helical coat protein and DNA with paraxial phosphates.
Science, 265:671-674, 1994

PubMed Abstract: The helical path of the DNA in filamentous bacteriophage Pf1 was deduced from different kinds of existing structural information, including results from x-ray fiber diffraction. The DNA has the same pitch, 16 angstroms, as the surrounding helix of protein subunits; the rise and rotation per nucleotides are 6.1 angstroms and 132 degrees, respectively; and the phosphates are 2.5 angstroms from the axis. The DNA in Pf1 is, therefore, the most extended and twisted DNA structure known. On the basis of the DNA structure and extensive additional information about the protein, a model of the virion is proposed. In the model, the DNA bases reach out, into the protein, and the lysine and arginine side chains reach in, between the DNA bases, to stabilize the paraxial phosphate charges; the conformation of the protein subunit is a combination of alpha and 3(10) helices.

PubMed: 8036516

実験手法

FIBER DIFFRACTION (3 Å)