1PC2

Solution structure of human mitochondria fission protein Fis1

1PC2 の概要

• エントリーDOI: 10.2210/pdb1pc2/pdb
• 分子名称: mitochondria fission protein (1 entity in total)
• 機能のキーワード: mitochondria, fission, unknown function
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Mitochondrion outer membrane; Single-pass membrane protein: Q9Y3D6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17229.89

構造登録者

Suzuki, M.,Youle, R.J.,Tjandra, N. (登録日: 2003-05-15, 公開日: 2003-12-09, 最終更新日: 2024-05-29)

主引用文献

Suzuki, M.,Jeong, S.Y.,Karbowski, M.,Youle, R.J.,Tjandra, N.
The Solution Structure of Human Mitochondria Fission Protein Fis1 Reveals a Novel TPR-like Helix Bundle
J.Mol.Biol., 334:445-458, 2003

PubMed Abstract: Fis1 in yeast localizes to the outer mitochondrial membrane and facilitates mitochondrial fission by forming protein complexes with Dnm1 and Mdv1. Fis1 orthologs exist in higher eukaryotes, suggesting that they are functionally conserved. In the present study, we cloned the human Fis1 ortholog that was predicted in a database, and determined the protein structure using NMR spectroscopy. Following a flexible N-terminal tail, six alpha-helices connected with short loops construct a single core domain. The C-terminal tail containing a transmembrane segment appears to be disordered. In the core domain, each of two sequentially adjacent helices forms a hairpin-like conformation, resulting in a six helix assembly forming a slightly twisted slab similar to that of a tandem array of tetratrico-peptide repeat (TPR) motif folds. Within this TPR-like core domain, no significant sequence similarity to the typical TPR motif is found. The structural analogy to the TPR-containing proteins suggests that Fis1 binds to other proteins at its concave hydrophobic surface. A simple composition of Fis1 comprised of a binding domain and a transmembrane segment indicates that the protein may function as a molecular adaptor on the mitochondrial outer membrane. In HeLa cells, however, increased levels in mitochondria-associated Fis1 did not result in mitochondrial translocation of Drp1, a potential binding partner of Fis1 implicated in the regulation of mitochondrial fission, suggesting that the interaction between Drp1 and Fis1 is regulated.

PubMed: 14623186
DOI: 10.1016/j.jmb.2003.09.064

実験手法

SOLUTION NMR