1PBZ

DE NOVO DESIGNED PEPTIDE-METALLOPORPHYRIN COMPLEX, SOLUTION STRUCTURE

1PBZ の概要

• エントリーDOI: 10.2210/pdb1pbz/pdb
• 分子名称: De novo designed cyclic peptide, COPROPORPHYRIN I CONTAINING CO(III) (2 entities in total)
• 機能のキーワード: peptide; metalloporphyrin; heme; de novo design, de novo protein
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 3883.24

構造登録者

Wang, J.,Rosenblatt, M.M.,Suslick, K.S. (登録日: 2003-05-15, 公開日: 2003-12-09, 最終更新日: 2024-11-06)

主引用文献

Rosenblatt, M.M.,Wang, J.,Suslick, K.S.
De novo designed cyclic-peptide heme complexes
Proc.Natl.Acad.Sci.USA, 100:13140-13145, 2003

PubMed Abstract: The structural characterization of de novo designed metalloproteins together with determination of chemical reactivity can provide a detailed understanding of the relationship between protein structure and functional properties. Toward this goal, we have prepared a series of cyclic peptides that bind to water-soluble metalloporphyrins (FeIII and CoIII). Neutral and positively charged histidine-containing peptides bind with a high affinity, whereas anionic peptides bind only weakly to the negatively charged metalloporphyrin. Additionally, it was found that the peptide becomes helical only in the presence of the metalloporphyrin. CD experiments confirm that the metalloporphyrin binds specific cyclic peptides with high affinity and with isodichroic behavior. Thermal unfolding experiments show that the complex has "native-like" properties. Finally, NMR spectroscopy produced well dispersed spectra and experimental restraints that provide a high-resolution solution structure of the complexed peptide.

PubMed: 14595023
DOI: 10.1073/pnas.2231273100

実験手法

SOLUTION NMR