1PBR
STRUCTURE OF 16S RIBOSOMAL RNA, NMR, MINIMIZED AVERAGE STRUCTURE
Summary for 1PBR
| Entry DOI | 10.2210/pdb1pbr/pdb |
| Descriptor | 16S RIBOSOMAL RNA, 2,6-diamino-2,6-dideoxy-beta-L-idopyranose-(1-3)-beta-D-ribofuranose, 2-amino-2-deoxy-alpha-D-glucopyranose, ... (4 entities in total) |
| Functional Keywords | aminoglycoside, ribosomal rna, rna transcript, antibiotic, ribonucleic acid, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 9307.84 |
| Authors | Fourmy, D.,Recht, M.I.,Blanchard, S.,Puglisi, J.D. (deposition date: 1996-09-12, release date: 1997-09-17, Last modification date: 2024-05-01) |
| Primary citation | Fourmy, D.,Recht, M.I.,Blanchard, S.C.,Puglisi, J.D. Structure of the A site of Escherichia coli 16S ribosomal RNA complexed with an aminoglycoside antibiotic. Science, 274:1367-1371, 1996 Cited by PubMed Abstract: Aminoglycoside antibiotics that bind to 30S ribosomal A-site RNA cause misreading of the genetic code and inhibit translocation. The aminoglycoside antibiotic paromomycin binds specifically to an RNA oligonucleotide that contains the 30S subunit A site, and the solution structure of the RNA-paromomycin complex was determined by nuclear magnetic resonance spectroscopy. The antibiotic binds in the major groove of the model A-site RNA within a pocket created by an A-A base pair and a single bulged adenine. Specific interactions occur between aminoglycoside chemical groups important for antibiotic activity and conserved nucleotides in the RNA. The structure explains binding of diverse aminoglycosides to the ribosome, their specific activity against prokaryotic organisms, and various resistance mechanisms, and provides insight into ribosome function. PubMed: 8910275DOI: 10.1126/science.274.5291.1367 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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