1PBR

STRUCTURE OF 16S RIBOSOMAL RNA, NMR, MINIMIZED AVERAGE STRUCTURE

1PBR の概要

• エントリーDOI: 10.2210/pdb1pbr/pdb
• 分子名称: 16S RIBOSOMAL RNA, 2,6-diamino-2,6-dideoxy-beta-L-idopyranose-(1-3)-beta-D-ribofuranose, 2-amino-2-deoxy-alpha-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: aminoglycoside, ribosomal rna, rna transcript, antibiotic, ribonucleic acid, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9307.84

構造登録者

Fourmy, D.,Recht, M.I.,Blanchard, S.,Puglisi, J.D. (登録日: 1996-09-12, 公開日: 1997-09-17, 最終更新日: 2024-05-01)

主引用文献

Fourmy, D.,Recht, M.I.,Blanchard, S.C.,Puglisi, J.D.
Structure of the A site of Escherichia coli 16S ribosomal RNA complexed with an aminoglycoside antibiotic.
Science, 274:1367-1371, 1996

PubMed Abstract: Aminoglycoside antibiotics that bind to 30S ribosomal A-site RNA cause misreading of the genetic code and inhibit translocation. The aminoglycoside antibiotic paromomycin binds specifically to an RNA oligonucleotide that contains the 30S subunit A site, and the solution structure of the RNA-paromomycin complex was determined by nuclear magnetic resonance spectroscopy. The antibiotic binds in the major groove of the model A-site RNA within a pocket created by an A-A base pair and a single bulged adenine. Specific interactions occur between aminoglycoside chemical groups important for antibiotic activity and conserved nucleotides in the RNA. The structure explains binding of diverse aminoglycosides to the ribosome, their specific activity against prokaryotic organisms, and various resistance mechanisms, and provides insight into ribosome function.

PubMed: 8910275
DOI: 10.1126/science.274.5291.1367

実験手法

SOLUTION NMR