1PA4

Solution structure of a putative ribosome-binding factor from Mycoplasma pneumoniae (MPN156)

1PA4 の概要

• エントリーDOI: 10.2210/pdb1pa4/pdb
• 関連するPDBエントリー: 1KKG
• NMR情報: BMRB: 5796
• 分子名称: Probable ribosome-binding factor A (1 entity in total)
• 機能のキーワード: ribosome-binding factor, structural genomics, distant homology, bsgc structure funded by nih, protein structure initiative, psi, berkeley structural genomics center, unknown function
• 由来する生物種: Mycoplasma pneumoniae
• 細胞内の位置: Cytoplasm (Potential): P75589
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13413.53

構造登録者

Rubin, S.M.,Pelton, J.G.,Yokota, H.,Kim, R.,Wemmer, D.E.,Berkeley Structural Genomics Center (BSGC) (登録日: 2003-05-13, 公開日: 2004-03-02, 最終更新日: 2024-05-22)

主引用文献

Rubin, S.M.,Pelton, J.G.,Yokota, H.,Kim, R.,Wemmer, D.E.
Solution structure of a putative ribosome binding protein from Mycoplasma pneumoniae and comparison to a distant homolog.
J.STRUCT.FUNCT.GENOM., 4:235-243, 2003

PubMed Abstract: The solution structure of MPN156, a ribosome-binding factor A (RBFA) protein family member from Mycoplasma pneumoniae, is presented. The structure, solved by nuclear magnetic resonance, has a type II KH fold typical of RNA binding proteins. Despite only approximately 20% sequence identity between MPN156 and another family member from Escherichia coli, the two proteins have high structural similarity. The comparison demonstrates that many of the conserved residues correspond to conserved elements in the structures. Compared to a structure based alignment, standard alignment methods based on sequence alone mispair a majority of amino acids in the two proteins. Implications of these discrepancies for sequence based structural modeling are discussed.

PubMed: 15185964
DOI: 10.1023/B:JSFG.0000016127.57320.82

実験手法

SOLUTION NMR