1P9F

NMR Structure of Neurokinin B from DYANA

1P9F の概要

• エントリーDOI: 10.2210/pdb1p9f/pdb
• NMR情報: BMRB: 5864
• 分子名称: NEUROKININ B (1 entity in total)
• 機能のキーワード: protein, structures from dyana, neuropeptide
• 細胞内の位置: Secreted: Q9UHF0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 1212.42

構造登録者

Mantha, A.K.,Chandrashekar, I.R.,Baquer, N.Z.,Cowsik, S.M. (登録日: 2003-05-12, 公開日: 2004-08-10, 最終更新日: 2024-05-22)

主引用文献

Mantha, A.K.,Chandrashekar, I.R.,Baquer, N.Z.,Cowsik, S.M.
Three dimensional structure of Mammalian tachykinin Peptide neurokinin B bound to lipid micelles.
J.Biomol.Struct.Dyn., 22:137-148, 2004

PubMed Abstract: Neurokinin B (NKB), a decapeptide of mammalian origin exhibits a variety of biological activities such as regulatory functions in reproduction, pre-eclampsia and neuroprotection in Alzheimer's disease. In order to gain insight into structure-function relationship, three-dimensional structure of NKB has been investigated using CD spectropolarimetry and two-dimensional proton nuclear magnetic resonance (2D 1H-NMR) spectroscopy in aqueous and membrane mimetic solvents. Unambiguous NMR assignments of resonances have been made with the aid of correlation spectroscopy (DQF-COSY and TOCSY) experiments and Nuclear Overhauser Effect Spectroscopy (NOESY) experiments. Distance constraints obtained from the NMR data have been used to generate a family of structures, which have been refined using restrained energy minimization and dynamics. Our data show that a helical structure is induced in NKB, in presence of perdeuterated dodecyl phosphocholine (DPC) micelles, a membrane model system. Further, the conformation adopted by NKB in presence of DPC micelles represents a structural motif typical of neurokinin-3 selective agonists.

PubMed: 15317475

実験手法

SOLUTION NMR