1P7I

CRYSTAL STRUCTURE OF ENGRAILED HOMEODOMAIN MUTANT K52A

1P7I の概要

• エントリーDOI: 10.2210/pdb1p7i/pdb
• 関連するPDBエントリー: 1DUO 1ENH 2HDD 3HDD
• 分子名称: Segmentation polarity homeobox protein engrailed, 2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID (3 entities in total)
• 機能のキーワード: dna binding protein
• 由来する生物種: Drosophila melanogaster (fruit fly)
• 細胞内の位置: Nucleus: P02836
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 28867.95

構造登録者

Stollar, E.J.,Mayor, U.,Lovell, S.C.,Federici, L.,Freund, S.M.,Fersht, A.R.,Luisi, B.F. (登録日: 2003-05-02, 公開日: 2003-10-14, 最終更新日: 2023-08-16)

主引用文献

Stollar, E.J.,Mayor, U.,Lovell, S.C.,Federici, L.,Freund, S.M.,Fersht, A.R.,Luisi, B.F.
Crystal Structures of Engrailed Homeodomain Mutants: IMPLICATIONS FOR STABILITY AND DYNAMICS
J.Biol.Chem., 278:43699-43708, 2003

PubMed Abstract: We report the crystal structures and biophysical characterization of two stabilized mutants of the Drosophila Engrailed homeodomain that have been engineered to minimize electrostatic repulsion. Four independent copies of each mutant occupy the crystal lattice, and comparison of these structures illustrates variation that can be partly ascribed to networks of correlated conformational adjustments. Central to one network is leucine 26 (Leu26), which occupies alternatively two side chain rotameric conformations (-gauche and trans) and different positions within the hydrophobic core. Similar sets of conformational substates are observed in other Engrailed structures and in another homeodomain. The pattern of structural adjustments can account for NMR relaxation data and sequence co-variation networks in the wider homeodomain family. It may also explain the dysfunction associated with a P26L mutation in the human ARX homeodomain protein. Finally, we observe a novel dipolar interaction between a conserved tryptophan and a water molecule positioned along the normal to the indole ring. This interaction may explain the distinctive fluorescent properties of the homeodomain family.

PubMed: 12923178
DOI: 10.1074/jbc.M308029200

実験手法

X-RAY DIFFRACTION (2.1 Å)