1P78

Anabaena HU-DNA cocrystal structure (AHU2)

1P78 の概要

• エントリーDOI: 10.2210/pdb1p78/pdb
• 関連するPDBエントリー: 1B8Z 1IHF 1P51 1P71
• 分子名称: 5'-D(*TP*GP*CP*AP*TP*AP*TP*CP*AP*AP*TP*TP*TP*GP*TP*TP*GP*CP*AP*CP*C)-3', DNA-binding protein HU (3 entities in total)
• 機能のキーワード: protein-dna complex, dna bending, hu, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Anabaena sp.; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 32955.46

構造登録者

Swinger, K.S.,Lemberg, K.M.,Zhang, Y.,Rice, P.A. (登録日: 2003-04-30, 公開日: 2003-05-13, 最終更新日: 2023-08-16)

主引用文献

Swinger, K.S.,Lemberg, K.M.,Zhang, Y.,Rice, P.A.
Flexible DNA bending in HU-DNA cocrystal structures
Embo J., 22:3749-3760, 2003

PubMed Abstract: HU and IHF are members of a family of prokaryotic proteins that interact with the DNA minor groove in a sequence-specific (IHF) or non-specific (HU) manner to induce and/or stabilize DNA bending. HU plays architectural roles in replication initiation, transcription regulation and site-specific recombination, and is associated with bacterial nucleoids. Cocrystal structures of Anabaena HU bound to DNA (1P71, 1P78, 1P51) reveal that while underlying proline intercalation and asymmetric charge neutralization mechanisms of DNA bending are similar for IHF and HU, HU stabilizes different DNA bend angles ( approximately 105-140 degrees ). The two bend angles within a single HU complex are not coplanar, and the resulting dihedral angle is consistent with negative supercoiling. Comparison of HU-DNA and IHF-DNA structures suggests that sharper bending is correlated with longer DNA binding sites and smaller dihedral angles. An HU-induced bend may be better modeled as a hinge, not a rigid bend. The ability to induce or stabilize varying bend angles is consistent with HU's role as an architectural cofactor in many different systems that may require differing geometries.

PubMed: 12853489
DOI: 10.1093/emboj/cdg351

実験手法

X-RAY DIFFRACTION (2.25 Å)