1P5M

Solution Structure of HCV IRES Domain IIa

1P5M の概要

• エントリーDOI: 10.2210/pdb1p5m/pdb
• 分子名称: 55-MER (1 entity in total)
• 機能のキーワード: ribonucleic acid, hepatitis c virus, internal ribosome entry site, trna, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17625.43

構造登録者

Lukavsky, P.J.,Kim, I.,Otto, G.A.,Puglisi, J.D. (登録日: 2003-04-27, 公開日: 2003-11-04, 最終更新日: 2024-05-22)

主引用文献

Lukavsky, P.J.,Kim, I.,Otto, G.A.,Puglisi, J.D.
Structure of HCV IRES domain II determined by NMR.
Nat.Struct.Biol., 10:1033-1038, 2003

PubMed Abstract: Complex RNA structures regulate many biological processes, but are often too large for structure determination by NMR methods. The 5' untranslated region (5' UTR) of the hepatitis C viral (HCV) RNA genome contains an internal ribosome entry site (IRES) that binds to 40S ribosomal subunits with high affinity and specificity to control translation. Domain II of the HCV IRES forms a 25-kDa folded subdomain that may alter ribosome conformation. We report here the structure of domain II as determined using an NMR approach that combines short- and long-range structural data. Domain II adopts a distorted L-shape structure, and its overall shape in the free form is markedly similar to its 40S subunit-bound form; this suggests how domain II may modulate 40S subunit conformation. The results show how NMR can be used for structural analysis of large biological RNAs.

PubMed: 14578934
DOI: 10.1038/nsb1004

実験手法

SOLUTION NMR