1P54

Effect of Sequence on the Conformational Geometry of DNA Holliday Junctions

1P54 の概要

• エントリーDOI: 10.2210/pdb1p54/pdb
• 関連するPDBエントリー: 1P4Y 1P4Z
• 分子名称: 5'-D(*CP*CP*AP*GP*TP*AP*CP*(BRU)P*GP*G)-3', SODIUM ION, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: dna holliday junction, four-way junction, stacked-x junction, brominated uracil, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6282.82

構造登録者

Hays, F.A.,Vargason, J.M.,Ho, P.S. (登録日: 2003-04-25, 公開日: 2003-09-02, 最終更新日: 2023-08-16)

主引用文献

Hays, F.A.,Vargason, J.M.,Ho, P.S.
Effect of Sequence on the Conformation of DNA Holliday Junctions
Biochemistry, 42:9586-9597, 2003

PubMed Abstract: Structures of the DNA sequences d(CCGGCGCCGG) and d(CCAGTACbr(5)UGG) are presented here as four-way Holliday junctions in their compact stacked-X forms, with antiparallel alignment of the DNA strands. Thus, the ACC-trinucleotide motif, previously identified as important for stabilizing the junction, is now extended to PuCPy, where Pu is either an adenine or guanine, and Py is either a cytosine, 5-methylcytosine, or 5-bromouracil but not thymine nucleotide. We see that both sequence and base substituents affect the geometry of the junction in terms of the interduplex angle as well as the previously defined conformational variables, J(roll) (the rotation of the stacked duplexes about their respective helical axis) and J(slide) (the translational displacement of the stacked duplexes along their respective helical axis). The structures of the GCC and parent ACC containing junctions fall into a distinct conformational class that is relatively undistorted in terms of J(slide) and J(roll), with interduplex angles of 40-43 degrees. The substituted ACbr(5)U structure, however, is more akin to that of the distorted methylated ACm(5)C containing junction, with J(slide) (>or=2.3 A) and a similar J(roll) (164 degrees) opening the major groove-side of the junction, but shows a reduced interduplex angle. In contrast, the analogous d(CCAGTACTGG) sequence has to date been crystallized only as resolved B-DNA duplexes. This suggests that there is an electronic effect of substituents at the pyrimidine Py position on the stability of four-stranded junctions. The single-crystal structures presented here, therefore, show how sequence affects the detailed geometry, and subsequently, the associated stability and conformational dynamics of the Holliday junction.

PubMed: 12911300
DOI: 10.1021/bi0346603

実験手法

X-RAY DIFFRACTION (1.9 Å)