1P3J

Adenylate Kinase from Bacillus subtilis

1P3J の概要

• エントリーDOI: 10.2210/pdb1p3j/pdb
• 分子名称: Adenylate kinase, ZINC ION, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: zinc coordination, transferase
• 由来する生物種: Bacillus subtilis
• 細胞内の位置: Cytoplasm: P16304
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25153.48

構造登録者

Bae, E.,Phillips Jr., G.N. (登録日: 2003-04-17, 公開日: 2004-05-04, 最終更新日: 2023-08-16)

主引用文献

Bae, E.,Phillips Jr., G.N.
Structures and analysis of highly homologous psychrophilic, mesophilic, and thermophilic adenylate kinases.
J.Biol.Chem., 279:28202-28208, 2004

PubMed Abstract: The crystal structures of adenylate kinases from the psychrophile Bacillus globisporus and the mesophile Bacillus subtilis have been solved and compared with that from the thermophile Bacillus stearothermophilus. This is the first example we know of where a trio of protein structures has been solved that have the same number of amino acids and a high level of identity (66-74%) and yet come from organisms with different operating temperatures. The enzymes were characterized for their own thermal denaturation and inactivation, and they exhibited the same temperature preferences as their source organisms. The structures of the three highly homologous, dynamic proteins with different temperature-activity profiles provide an opportunity to explore a molecular mechanism of cold and heat adaptation. Their analysis suggests that the maintenance of the balance between stability and flexibility is crucial for proteins to function at their environmental temperatures, and it is achieved by the modification of intramolecular interactions in the process of temperature adaptation.

PubMed: 15100224
DOI: 10.1074/jbc.M401865200

実験手法

X-RAY DIFFRACTION (1.9 Å)