1P2Z

Refinement of Adenovirus Type 2 Hexon with CNS

「1DHX」から置き換えられました

1P2Z の概要

• エントリーDOI: 10.2210/pdb1p2z/pdb
• 関連するPDBエントリー: 1DHX 1p30 1RUX
• 分子名称: Hexon protein, CITRIC ACID (3 entities in total)
• 機能のキーワード: adenovirus, type 2, hexon, virus, jellyroll, coat protein, viral protein
• 由来する生物種: Human adenovirus 2
• 細胞内の位置: Virion : P03277
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 110081.75

構造登録者

Rux, J.J.,Kuser, P.R.,Burnett, R.M. (登録日: 2003-04-16, 公開日: 2003-11-11, 最終更新日: 2023-08-16)

主引用文献

Rux, J.J.,Kuser, P.R.,Burnett, R.M.
Structural and phylogenetic analysis of adenovirus hexons by use of high-resolution x-ray crystallographic, molecular modeling, and sequence-based methods
J.VIROL., 77:9553-9566, 2003

PubMed Abstract: A major impediment to the use of adenovirus as a gene therapy vector and for vaccine applications is the host immune response to adenovirus hexon-the major protein component of the icosahedral capsid. A solution may lie in novel vectors with modified or chimeric hexons designed to evade the immune response. To facilitate this approach, we have distinguished the portion of hexon that all serotypes have in common from the hypervariable regions that are responsible for capsid diversity and type-specific immunogenicity. The common hexon core-conserved because it forms the viral capsid-sets boundaries to the regions where modifications can be made to produce nonnative hexons. The core has been defined from the large and diverse set of known hexon sequences by an accurate alignment based on the newly refined crystal structures of human adenovirus types 2 (Ad2) and Ad5 hexon. Comparison of the two hexon models, which are the most accurate so far, reveals that over 90% of the residues in each have three-dimensional positions that closely match. Structures for more distant hexons were predicted by building molecular models of human Ad4, chimpanzee adenovirus (AdC68), and fowl adenovirus 1 (FAV1 or CELO). The five structures were then used to guide the alignment of the 40 full-length (>900 residues) hexon sequences in public databases. Distance- and parsimony-based phylogenetic trees are consistent and reveal evolutionary relationships between adenovirus types that parallel those of their animal hosts. The combination of crystallography, molecular modeling, and phylogenetic analysis defines a conserved molecular core that can serve as the armature for the directed design of novel hexons.

PubMed: 12915569
DOI: 10.1128/JVI.77.17.9553-9566.2003

実験手法

X-RAY DIFFRACTION (2.2 Å)