1P24

Crystal structure of cobalt(II)-d(GGCGCC)2

1P24 の概要

• エントリーDOI: 10.2210/pdb1p24/pdb
• 関連するPDBエントリー: 1P25 1P26
• 分子名称: DNA (5'-D(*GP*GP*CP*GP*CP*C)-3'), COBALT (II) ION (3 entities in total)
• 機能のキーワード: b-dna, cobalt binding, dna
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 9345.69

構造登録者

Labiuk, S.L.,Delbaere, L.T.,Lee, J.S. (登録日: 2003-04-14, 公開日: 2003-12-09, 最終更新日: 2024-04-03)

主引用文献

Labiuk, S.L.,Delbaere, L.T.,Lee, J.S.
Cobalt(II), nickel(II) and zinc(II) do not bind to intra-helical N(7) guanine positions in the B-form crystal structure of d(GGCGCC)
J.Biol.Inorg.Chem., 8:715-720, 2003

PubMed Abstract: Three novel X-ray crystal structures for the DNA hexamer d(GGCGCC) in the B-form complexed to divalent cobalt, nickel and zinc ions have been determined to a resolution of 2.9-3.0 A. The structures were isomorphous and had five DNA strands and five metal cations per asymmetric unit. In all three cases, divalent metal cations were coordinated only to the terminal guanine residue at the N(7) position, with no metal ions binding to non-terminal guanine positions. Water molecules bound to the metal cations interacted with neighboring guanine residues 3' to the ones to which the cations were coordinated, affecting the propeller twist. Even though DNA occupied only about 35% of the unit cell volume, it is interesting that the few interactions involving the metal cations were sufficient to stabilize the crystal lattice. As well as lending support to the proposal that these metals do not coordinate to B-DNA in a stable manner, the results presented here also extend the crystallographic evidence for this phenomenon to the GGC and CGC sequences for all three metal cations.

PubMed: 14505075
DOI: 10.1007/s00775-003-0473-4

実験手法

X-RAY DIFFRACTION (3.02 Å)