1OZI

The alternatively spliced PDZ2 domain of PTP-BL

1OZI の概要

• エントリーDOI: 10.2210/pdb1ozi/pdb
• 関連するPDBエントリー: 1GM1
• 分子名称: protein tyrosine phosphatase (1 entity in total)
• 機能のキーワード: all-beta protein, pdz domain, interaction with c-termini, apc, adenomatous polyposis coli, ril, reversion induced lim, hydrolase
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cytoplasm, cytoskeleton (By similarity): Q64512
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10370.79

構造登録者

Walma, T.,Aelen, J.,Oostendorp, M.,van den Berk, L.,Hendriks, W.,Vuister, G.W. (登録日: 2003-04-09, 公開日: 2004-01-27, 最終更新日: 2024-05-22)

主引用文献

Walma, T.,Aelen, J.,Nabuurs, S.B.,Oostendorp, M.,van den Berk, L.,Hendriks, W.,Vuister, G.W.
A Closed Binding Pocket and Global Destabilization Modify the Binding Properties of an Alternatively Spliced Form of the Second PDZ Domain of PTP-BL.
Structure, 12:11-20, 2004

PubMed Abstract: PTP-BL is a large phosphatase that is implicated in cellular processes as diverse as cytokinesis, actin-cytoskeletal rearrangement, and apoptosis. Five PDZ domains mediate its cellular role by binding to the C termini of target proteins, forming multiprotein complexes. The second PDZ domain (PDZ2) binds to the C termini of the tumor suppressor protein APC and the LIM domain-containing protein RIL; however, in one splice variant, PDZ2as, a 5 residue insertion abrogates this binding. The insert causes distinct structural and dynamical changes in the alternatively spliced PDZ2: enlarging the L1 loop between beta2 and beta3, both lengthening and changing the orientation of the alpha2 helix, giving the base of the binding pocket less flexibility to accommodate ligands, and destabilizing the entire domain. These changes render the binding pocket incapable of binding C termini, possibly having implications in the functional role of PTP-BL.

PubMed: 14725761
DOI: 10.1016/j.str.2003.11.023

実験手法

SOLUTION NMR