1OZ9

Crystal structure of AQ_1354, a hypothetical protein from Aquifex aeolicus

1OZ9 の概要

• エントリーDOI: 10.2210/pdb1oz9/pdb
• 分子名称: Hypothetical protein AQ_1354 (2 entities in total)
• 機能のキーワード: matrix metalloproteinase type fold, structural genomics, bsgc structure funded by nih, protein structure initiative, psi, berkeley structural genomics center, unknown function
• 由来する生物種: Aquifex aeolicus
• 細胞内の位置: Cytoplasm (By similarity): O67367
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17372.02

構造登録者

Oganesyan, V.,Busso, D.,Brandsen, J.,Chen, S.,Jancarik, J.,Kim, R.,Kim, S.H.,Berkeley Structural Genomics Center (BSGC) (登録日: 2003-04-08, 公開日: 2003-09-23, 最終更新日: 2024-02-14)

主引用文献

Oganesyan, V.,Busso, D.,Brandsen, J.,Chen, S.,Jancarik, J.,Kim, R.,Kim, S.H.
Structure of the hypothetical protein AQ_1354 from Aquifex aeolicus.
Acta Crystallogr.,Sect.D, 59:1219-1223, 2003

PubMed Abstract: The crystal structure of a hypothetical protein AQ_1354 (gi 2983779) from the hyperthermophilic bacteria Aquifex aeolicus has been determined using X-ray crystallography. As found in many structural genomics studies, this protein is not associated with any known function based on its amino-acid sequence. PSI-BLAST analysis against a non-redundant sequence database gave 68 similar sequences referred to as 'conserved hypothetical proteins' from the uncharacterized protein family UPF0054 (accession No. PF02310). Crystallographic analysis revealed that the overall fold of this protein consists of one central alpha-helix surrounded by a four-stranded beta-sheet and four other alpha-helices. Structure-based homology analysis with DALI revealed that the structure has a moderate to good resemblance to metal-dependent proteinases such as collagenases and gelatinases, thus suggesting its possible molecular function. However, experimental tests for collagenase and gelatinase-type function show no detectable activity under standard assay conditions. Therefore, we suggest either that the members of the UPF0054 family have a similar fold but different biochemical functions to those of collagenases and gelatinases or that they have a similar function but perform it under different conditions.

PubMed: 12832766
DOI: 10.1107/S0907444903011028

実験手法

X-RAY DIFFRACTION (1.894 Å)