1OZ8
Intramolecular higher-order packing of parallel quadruplexes comprising a G:G:G:G tetrad and a G(:A):G(:A):G(:A):G heptad of GGA triplet repeat DNA
Summary for 1OZ8
| Entry DOI | 10.2210/pdb1oz8/pdb |
| Descriptor | 5'-D(*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*A)-3' (1 entity in total) |
| Functional Keywords | intramolecular packing of parallel quadruplexes, dna |
| Total number of polymer chains | 1 |
| Total formula weight | 7727.99 |
| Authors | Matsugami, A.,Okuizumi, T.,Uesugi, S.,Katahira, M. (deposition date: 2003-04-08, release date: 2004-04-08, Last modification date: 2024-05-29) |
| Primary citation | Matsugami, A.,Okuizumi, T.,Uesugi, S.,Katahira, M. Intramolecular Higher Order Packing of Parallel Quadruplexes Comprising a G:G:G:G Tetrad and a G(:A):G(:A):G(:A):G Heptad of GGA Triplet Repeat DNA J.BIOL.CHEM., 278:28147-28153, 2003 Cited by PubMed Abstract: GGA triplet repeats are widely dispersed throughout eukaryotic genomes and are frequently located within biologically important regions such as gene regulatory regions and recombination hot spot sites. We determined the structure of d(GGA)4 (12-mer) under physiological conditions and founded the formation of an intramolecular parallel quadruplex for the first time. Later, a similar architecture to that of the intramolecular parallel quadruplex was found for a telomere DNA in the crystalline state. Here, we have determined the structure of d(GGA)8 (24-mer) under physiological conditions. Two intramolecular parallel quadruplexes comprising a G:G:G:G tetrad and a G(:A):G(:A):G(:A):G heptad are formed in d(GGA)8. These quadruplexes are packed in a tail-to-tail manner. This is the first demonstration of the intramolecular higher order packing of quadruplexes at atomic resolution. K+ ions, but not Na+ ones, are critically required for the formation of this unique structure. The elucidated structure suggests the mechanisms underlying the biological events related to the GGA triplet repeat. Furthermore, in the light of the structure, the mode of the higher order packing of the telomere DNA is discussed. PubMed: 12748183DOI: 10.1074/jbc.M303694200 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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