1OZ8

Intramolecular higher-order packing of parallel quadruplexes comprising a G:G:G:G tetrad and a G(:A):G(:A):G(:A):G heptad of GGA triplet repeat DNA

1OZ8 の概要

• エントリーDOI: 10.2210/pdb1oz8/pdb
• 分子名称: 5'-D(*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*A)-3' (1 entity in total)
• 機能のキーワード: intramolecular packing of parallel quadruplexes, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7727.99

構造登録者

Matsugami, A.,Okuizumi, T.,Uesugi, S.,Katahira, M. (登録日: 2003-04-08, 公開日: 2004-04-08, 最終更新日: 2024-05-29)

主引用文献

Matsugami, A.,Okuizumi, T.,Uesugi, S.,Katahira, M.
Intramolecular Higher Order Packing of Parallel Quadruplexes Comprising a G:G:G:G Tetrad and a G(:A):G(:A):G(:A):G Heptad of GGA Triplet Repeat DNA
J.BIOL.CHEM., 278:28147-28153, 2003

PubMed Abstract: GGA triplet repeats are widely dispersed throughout eukaryotic genomes and are frequently located within biologically important regions such as gene regulatory regions and recombination hot spot sites. We determined the structure of d(GGA)4 (12-mer) under physiological conditions and founded the formation of an intramolecular parallel quadruplex for the first time. Later, a similar architecture to that of the intramolecular parallel quadruplex was found for a telomere DNA in the crystalline state. Here, we have determined the structure of d(GGA)8 (24-mer) under physiological conditions. Two intramolecular parallel quadruplexes comprising a G:G:G:G tetrad and a G(:A):G(:A):G(:A):G heptad are formed in d(GGA)8. These quadruplexes are packed in a tail-to-tail manner. This is the first demonstration of the intramolecular higher order packing of quadruplexes at atomic resolution. K+ ions, but not Na+ ones, are critically required for the formation of this unique structure. The elucidated structure suggests the mechanisms underlying the biological events related to the GGA triplet repeat. Furthermore, in the light of the structure, the mode of the higher order packing of the telomere DNA is discussed.

PubMed: 12748183
DOI: 10.1074/jbc.M303694200

実験手法

SOLUTION NMR