1OXD

Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins

1OXD の概要

• エントリーDOI: 10.2210/pdb1oxd/pdb
• 関連するPDBエントリー: 1EMB
• 分子名称: cyan fluorescent protein cfp (2 entities in total)
• 機能のキーワード: green fluorescent protein, chromophore, amino acid incorporation, tryptophan, genetic code, luminescent protein
• 由来する生物種: cfp marker plasmid pWM1009
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25798.10

構造登録者

Hyun Bae, J.,Rubini, M.,Jung, G.,Wiegand, G.,Seifert, M.H.,Azim, M.K.,Kim, J.S.,Zumbusch, A.,Holak, T.A.,Moroder, L.,Huber, R.,Budisa, N. (登録日: 2003-04-02, 公開日: 2003-12-02, 最終更新日: 2024-10-30)

主引用文献

Hyun Bae, J.,Rubini, M.,Jung, G.,Wiegand, G.,Seifert, M.H.,Azim, M.K.,Kim, J.S.,Zumbusch, A.,Holak, T.A.,Moroder, L.,Huber, R.,Budisa, N.
Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins
J.Mol.Biol., 328:1071-1081, 2003

PubMed Abstract: Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora (av). These approaches have been based on classical engineering with the 20 canonical amino acids. We report here an expansion of these efforts by incorporation of an amino substituted variant of tryptophan into the "cyan" GFP mutant, which turned it into a "gold" variant. This variant possesses a red shift in emission unprecedented for any avFP, similar to "red" FPs, but with enhanced stability and a very low aggregation tendency. An increasing number of non-natural amino acids are available for chromophore redesign (by engineering of the genetic code) and enable new general strategies to generate novel classes of tailor-made GFP proteins.

PubMed: 12729742
DOI: 10.1016/S0022-2836(03)00364-4

実験手法

X-RAY DIFFRACTION (1.15 Å)