1OV3

Structure of the p22phox-p47phox complex

1OV3 の概要

• エントリーDOI: 10.2210/pdb1ov3/pdb
• 関連するPDBエントリー: 1NG2
• 分子名称: Neutrophil cytosol factor 1, Flavocytochrome b558 alpha polypeptide (3 entities in total)
• 機能のキーワード: p47phox, p22phox, nadph oxidase, complex, oxidoreductase activator
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: P14598
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 34792.92

構造登録者

Groemping, Y.,Lapouge, K.,Smerdon, S.J.,Rittinger, K. (登録日: 2003-03-25, 公開日: 2003-05-20, 最終更新日: 2023-09-20)

主引用文献

Groemping, Y.,Lapouge, K.,Smerdon, S.J.,Rittinger, K.
Molecular basis of phosphorylation-induced activation of the NADPH oxidase
Cell(Cambridge,Mass.), 113:343-355, 2003

PubMed Abstract: The multi-subunit NADPH oxidase complex plays a crucial role in host defense against microbial infection through the production of reactive oxygen species. Activation of the NADPH oxidase requires the targeting of a cytoplasmic p40-p47-p67(phox) complex to the membrane bound heterodimeric p22-gp91(phox) flavocytochrome. This interaction is prevented in the resting state due to an auto-inhibited conformation of p47(phox). The X-ray structure of the auto-inhibited form of p47(phox) reveals that tandem SH3 domains function together to maintain the cytoplasmic complex in an inactive form. Further structural and biochemical data show that phosphorylation of p47(phox) activates a molecular switch that relieves the inhibitory intramolecular interaction. This permits p47(phox) to interact with the cytoplasmic tail of p22(phox) and initiate formation of the active, membrane bound enzyme complex.

PubMed: 12732142
DOI: 10.1016/S0092-8674(03)00314-3

実験手法

X-RAY DIFFRACTION (1.8 Å)