1OUT

TROUT HEMOGLOBIN I

1OUT の概要

• エントリーDOI: 10.2210/pdb1out/pdb
• 分子名称: HEMOGLOBIN I, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• 機能のキーワード: heme, oxygen transport, respiratory protein, erythrocyte
• 由来する生物種: Oncorhynchus mykiss (rainbow trout); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32241.97

構造登録者

Tame, J.,Wilson, J. (登録日: 1996-06-21, 公開日: 1997-01-11, 最終更新日: 2024-10-23)

主引用文献

Tame, J.R.,Wilson, J.C.,Weber, R.E.
The crystal structures of trout Hb I in the deoxy and carbonmonoxy forms.
J.Mol.Biol., 259:749-760, 1996

PubMed Abstract: We have determined the X-ray crystallographic structure of trout Hb I in both the deoxy and carbonmonoxy forms to resolution limits of 2.3 angstroms and 2.5 angstroms, respectively. The overall fold of the molecule is highly similar to that of human HbA despite the low level of sequence identity between these proteins. Trout Hb I is unusual in displaying almost no pH dependence of oxygen binding affinity, and (at most) very weak interactions with heterotropic effector ligands such as organic phosphates. Comparison of the two quaternary states of the protein indicates how such effects are minimised and how the low-affinity T state of the protein is stabilised in the absence of heterotropic interactions.

PubMed: 8683580
DOI: 10.1006/jmbi.1996.0355

実験手法

X-RAY DIFFRACTION (2.3 Å)