1OUN

CRYSTAL STRUCTURE OF NUCLEAR TRANSPORT FACTOR 2 (NTF2)

1OUN の概要

• エントリーDOI: 10.2210/pdb1oun/pdb
• 分子名称: NUCLEAR TRANSPORT FACTOR 2 (2 entities in total)
• 機能のキーワード: transport, nuclear transport protein
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28982.85

構造登録者

Bullock, T.L.,Stewart, M.J. (登録日: 1996-02-21, 公開日: 1997-04-01, 最終更新日: 2024-02-14)

主引用文献

Bullock, T.L.,Clarkson, W.D.,Kent, H.M.,Stewart, M.
The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2).
J.Mol.Biol., 260:422-431, 1996

PubMed Abstract: Nuclear transport factor 2 (NTF2) facilitates protein transport into the nucleus and interacts with both the small Ras-like GTPase Ran and nucleoporin p62. We have determined the structure of bacterially expressed rat NTF2 at 1.6 angstroms resolution using X-ray crystallography. The NTF2 polypeptide chain forms an alpha + beta barrel that opens at one end to form a distinctive hydrophobic cavity and its fold is homologous to that of scytalone dehydratase. The NTF2 hydrophobic cavity is a candidate for a potential binding site for other proteins involved in nuclear import such as Ran and nucleoporin p62. In addition, the hydrophobic cavity contains a putative catalytic Asp-His pair, which raises the possibility of an unanticipated enzymatic activity of the molecule that may have implications for the molecular mechanism of nuclear protein import.

PubMed: 8757804
DOI: 10.1006/jmbi.1996.0411

実験手法

X-RAY DIFFRACTION (2.3 Å)