1ORX

Solution Structure of the acyclic permutant des-(24-28)-kalata B1.

1ORX の概要

• エントリーDOI: 10.2210/pdb1orx/pdb
• 関連するPDBエントリー: 1NB1 1NBJ
• 分子名称: kalata B1 (1 entity in total)
• 機能のキーワード: acyclic permutation, cyclotides, kalata b1, antibiotic
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2378.72

構造登録者

Barry, D.G.,Daly, N.L.,Clark, R.J.,Sando, L.,Craik, D.J. (登録日: 2003-03-17, 公開日: 2003-06-24, 最終更新日: 2024-10-09)

主引用文献

Barry, D.G.,Daly, N.L.,Clark, R.J.,Sando, L.,Craik, D.J.
Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity
Biochemistry, 42:6688-6695, 2003

PubMed Abstract: Cyclotides are a recently discovered family of disulfide rich proteins from plants that contain a circular protein backbone. They are exceptionally stable, as exemplified by their use in native medicine of the prototypic cyclotide kalata B1. The peptide retains uterotonic activity after the plant from which it is derived is boiled to make a medicinal tea. The circular backbone is thought to be in part responsible for the stability of the cyclotides, and to investigate its role in determining structure and biological activity, an acyclic derivative, des-(24-28)-kalata B1, was chemically synthesized and purified. This derivative has five residues removed from the 29-amino acid circular backbone of kalata B1 in a loop region corresponding to a processing site in the biosynthetic precursor protein. Two-dimensional NMR spectra of the peptide were recorded, assigned, and used to identify a series of distance, angle, and hydrogen bonding restraints. These were in turn used to determine a representative family of solution structures. Of particular interest was a determination of the structural similarities and differences between des-(24-28)-kalata B1 and native kalata B1. Although the overall three-dimensional fold remains very similar to that of the native circular protein, removal of residues 24-28 of kalata B1 causes disruption of some structural features that are important to the overall stability. Furthermore, loss of hemolytic activity is associated with backbone truncation and linearization.

PubMed: 12779323
DOI: 10.1021/bi027323n

実験手法

SOLUTION NMR