1ORP

Structure of a Trapped Endonuclease III-DNA Covalent Intermediate: Estranged-Adenine Complex

1ORP の概要

• エントリーDOI: 10.2210/pdb1orp/pdb
• 関連するPDBエントリー: 1ORN 2ABK
• 分子名称: 5'-D(*AP*AP*GP*AP*CP*AP*TP*GP*GP*AP*C)-3', 5'-D(*GP*TP*CP*CP*AP*(PED)P*GP*TP*CP*T)-3', Endonuclease III, ... (6 entities in total)
• 機能のキーワード: dna repair, dna glycosylase, [4fe-4s] cluster, iron-sulfur cluster, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 33193.78

構造登録者

Fromme, J.C.,Verdine, G.L. (登録日: 2003-03-14, 公開日: 2003-07-15, 最終更新日: 2024-11-20)

主引用文献

Fromme, J.C.,Verdine, G.L.
Structure of a Trapped Endonuclease III-DNA Covalent Intermediate
Embo J., 22:3461-3471, 2003

PubMed Abstract: Nearly all cells express proteins that confer resistance to the mutagenic effects of oxidative DNA damage. The primary defense against the toxicity of oxidative nucleobase lesions in DNA is the base-excision repair (BER) pathway. Endonuclease III (EndoIII) is a [4Fe-4S] cluster-containing DNA glycosylase with repair activity specific for oxidized pyrimidine lesions in duplex DNA. We have determined the crystal structure of a trapped intermediate that represents EndoIII frozen in the act of repairing DNA. The structure of the protein-DNA complex provides insight into the ability of EndoIII to recognize and repair a diverse array of oxidatively damaged bases. This structure also suggests a rationale for the frequent occurrence in certain human cancers of a specific mutation in the related DNA repair protein MYH.

PubMed: 12840008
DOI: 10.1093/emboj/cdg311

実験手法

X-RAY DIFFRACTION (2.2 Å)