1ORL

1H NMR structure determination of Viscotoxin C1

1ORL の概要

• エントリーDOI: 10.2210/pdb1orl/pdb
• 関連するPDBエントリー: 1ED0
• NMR情報: BMRB: 5767
• 分子名称: Viscotoxin C1 (1 entity in total)
• 機能のキーワード: helix-turn-helix, beta-sheet, concentric motif of disulphide bridges, toxin
• 由来する生物種: Viscum album (European mistletoe)
• 細胞内の位置: Secreted: P08943
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4954.69

構造登録者

Molinari, H.,Romagnoli, S.,Fogolari, F.,Catalano, M.,Urech, K.,Giannattasio, M.,Ragona, L. (登録日: 2003-03-14, 公開日: 2003-04-01, 最終更新日: 2024-10-16)

主引用文献

Romagnoli, S.,Fogolari, F.,Catalano, M.,Zetta, L.,Schaller, G.,Urech, K.,Giannattasio, M.,Ragona, L.,Molinari, H.
NMR solution structure of viscotoxin C1 from Viscum album species Coloratum ohwi: toward a structure-function analysis of viscotoxins.
Biochemistry, 42:12503-12510, 2003

PubMed Abstract: The high resolution three-dimensional structure of the newly discovered plant viscotoxin C1, from the Asiatic Viscum album ssp. Coloratum ohwi, has been determined in solution by (1)H NMR spectroscopy at pH 3.6 and 285 K. The viscotoxin C1-fold, consisting of a helix-turn-helix motif and a short stretch of an antiparralel beta-sheet is very similar to that found for the highly similar viscotoxins A2 and A3 and for other related thionins. Different functional properties of members of the thionin family are discussed here in light of the structural and electrostatic properties. Among the very homologous family of alpha- and beta-thionins, known for their antimicrobial activity, the viscotoxin subfamily differs from the other members because of its high toxicity against tumoral cells. Key residues for the modulation of viscotoxin cytotoxicity have been identified on the basis of sequence and structural alignment.

PubMed: 14580196
DOI: 10.1021/bi034762t

実験手法

SOLUTION NMR