1OR6

Crystal Structure of HemAT sensor domain from B.subtilis in the unliganded form

1OR6 の概要

• エントリーDOI: 10.2210/pdb1or6/pdb
• 関連するPDBエントリー: 1OR4
• 分子名称: Heme-based aerotactic transducer hemAT, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: globin fold, signaling protein
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 42915.16

構造登録者

Zhang, W.,Phillips Jr., G.N. (登録日: 2003-03-11, 公開日: 2003-09-23, 最終更新日: 2023-08-16)

主引用文献

Zhang, W.,Phillips, G.N.
Structure of the oxygen sensor in Bacillus subtilis: signal transduction of chemotaxis by control of symmetry.
Structure, 11:1097-1110, 2003

PubMed Abstract: Much is now known about chemotaxis signaling transduction for Escherichia coli and Salmonella typhimurium. The mechanism of chemotaxis of Bacillus subtilis is, in a sense, reversed. Attractant binding strengthens the activity of histidine kinase in B. subtilis, instead of an inhibition reaction. The HemAT from B. subtilis can detect oxygen and transmit the signal to regulatory proteins that control the direction of flagella rotation. We have determined the crystal structures of the HemAT sensor domain in liganded and unliganded forms at 2.15 A and 2.7 A resolution, respectively. The liganded structure reveals a highly symmetrical organization. Tyrosine70 shows distinct conformational changes on one subunit when ligands are removed. Our study suggests that disruption of the symmetry of HemAT plays an important role in initiating the chemotaxis signaling transduction cascade.

PubMed: 12962628
DOI: 10.1016/S0969-2126(03)00169-2

実験手法

X-RAY DIFFRACTION (2.71 Å)